Optimal conditions must be determined individually for each application.
The IDH1 antibody can be used to qualitatively identify the presence of IDH1 R132H protein mutation in FFPE tissue sections using IHC test methods.
For IHC applications: Dilute the concentrate as appropriate in antibody diluent, and then apply 150 µL to the slide, as indicated in the user's IHC protocol.
Liquid. In Tris buffer, pH 7.5, containing 1% BSA and <0.1% sodium azide.
Avoid freeze/thaw cycles.
Shipped on Blue Ice
Short Term Storage:
Long Term Storage:
IDH1 (Isocitrate Dehydrogenase 1) is an enzyme found in the cytoplasm and perixosomes. IDH1 is a metabolic gene that is often mutated in human cancer. It is involved in metabolic and cellular pathways, specifically the Krebs cycle. Mutations are frequently found in diffuse low-grade gliomas and secondary glioblastomas, but rarely in primary glioblastomas. The most notable mutation in this enzyme is replacing amino acid arginine with histidine at codon 132 (R132H).
Formalin-fixed paraffin-embedded glioblastoma tissue stained using Enzo’s IDH1 antibody (Prod. No. ENZ-ABS774). IHC staining was performed on an automated Leica BOND III.